Parameter Specification Method
Vitamin D2 125 to 463 μg/g* HPLC-DAD
Moisture ≤7% O'haus Scale
Water activity <0.5% AOAC 978.18
Ash ≤13.5% AOAC 945.46
Physical Parameters
Appearance Light to dark brown milled powder Organoleptic
Aroma Earthy Organoleptic
Taste Mushroom savoury, umami flavour Organoleptic
Proximate Analysis
Total Fat ≤4.5% AOAC 996.06
Total Carbohydrate ≤60% Calculation
Protein ≤40% AOAC 992.23
Microbiological Parameters
Standard plate count <5,000 CFU/g AOAC 966.23
Total yeast and mould count <100 CFU/g FDA-BAM, 7th ed.
Salmonella Negative in 25 g AOAC 2004.03
Staphylococcus aureus Negative in 10 g AOAC 975.55
Escherichia coli Negative in 10 g Current USP/NF, 62
Coliform <100 MPN/ g AOAC 966.24
Listeria spp. Negative in 25 g AOAC 2004.06
Aflatoxins (sum of B1, B2, G1 and G2) <4 μg/kg AOAC 991.31 (Mod.)
Aflatoxin B1 <2 μg/kg AOAC 991.31 (Mod.)
Heavy Metals
Arsenic ≤0.3 mg/kg USP 730
Lead (as Pb) ≤0.5 mg/kg USP 730
Mercury ≤0.1 mg/kg USP 730
Cadmium ≤0.5 mg/kg USP 730