| Definition |
The manufacturing process comprises 2 main phases: the first involving water extraction of the leaves of the Stevia rebaudiana Bertoni plant and preliminary purification of the extract by employing ion exchange chromatography to yield a steviol glycoside primary extract, and the second involving recrystallisation of the steviol glycosides from methanol or aqueous ethanol resulting in a final product containing not less than 95 % of the below identified 11 related steviol glycosides, in any combination and ratio.
The additive may contain residues of ion-exchange resins used in the manufacturing process. Several other related steviol glycosides that may be generated as a result of the production process, but do not occur naturally in the Stevia rebaudiana plant, have been identified in small amounts (0.10 to 0.37 % w/w). |
| Chemical name |
Steviolbioside: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid
Rubusoside: 13-β-D-glucopyranosyloxykaur-16-en-18-oic acid, β-D-glucopyranosyl ester
Dulcoside A: 13-[(2-O-α–L-rhamnopyranosyl-β–D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl ester
Stevioside: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl ester
Rebaudioside A: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D- glucopyranosyl ester
Rebaudioside B: 13-[(2-O-β–D-glucopyranosyl-3-O-β–D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid
Rebaudioside C: 13-[(2-O-α–L-rhamnopyranosyl-3-O-β–D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl ester
Rebaudioside D: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester
Rebaudioside E: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester
Rebaudioside F: 13[(2-O-β-D-xylofurananosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl ester
Rebaudioside M: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester |
| Trivial name, molecular formula and conversion factors |
Steviol, C20H30O3, conversion factor 1.00.
Steviolbioside, C32H50O13, conversion factor 0.50.
Rubusoside, C32H50O13, conversion factor 0.50.
Dulcoside A, C38H60O17, conversion factor 0.40.
Stevioside, C38H60O18, conversion factor 0.40.
Rebaudioside A, C44H70O23, conversion factor 0.33.
Rebaudioside B, C38H60O18, conversion factor 0.40.
Rebaudioside C, C44H70O20, conversion factor 0.34.
Rebaudioside D, C50H80O28, conversion factor 0.29.
Rebaudioside E, C44H70O23, conversion factor 0.33.
Rebaudioside F, C43H68O22, conversion factor 0.34.
Rebaudioside M, C56H90O33, conversion factor 0.25. |
| Trivial name, CAS number and molecular weight (g/mol) |
Steviol, (no CAS listed), 318.46.
Steviolbioside, 41093-60-1, 642.73.
Rubusoside, 64849-39-4, 642.73.
Dulcoside A, 64432-06-0, 788.87.
Stevioside, 57817-89-7, 804.88.
Rebaudioside A, 58543-16-1, 967.01.
Rebaudioside B, 58543-17-2, 804.88.
Rebaudioside C, 63550-99-2, 951.02.
Rebaudioside D, 63279-13-0, 1,129.15.
Rebaudioside E, 63279-14-1, 967.01.
Rebaudioside F, 438045-89-7, 936.99.
Rebaudioside M, 1220616-44-3, 1,291.30. |
| Assay |
Not less than 95 % steviolbioside, rubusoside, dulcoside A, stevioside, rebaudiosides A, B, C, D, E, F, and M, on the dried basis, in any combination and ratio. |
| Description |
White to light yellow powder, approximately between 200 and 350 times sweeter than sucrose (at 5 % sucrose equivalency). |
| Identification (Solubility) |
Freely soluble to slightly soluble in water. |
| Identification (pH) |
Between 4.5 and 7.0 (1 in 100 solution). |
| Purity (Total ash) |
Not more than 1 %. |
| Purity (Loss on drying) |
Not more than 6 % (105 °C, 2 h). |
| Purity (Residual solvent) |
Not more than 200 mg/kg methanol. |
| Purity (Residual solvent) |
Not more than 5,000 mg/kg ethanol. |
| Purity (Arsenic) |
Not more than 1 mg/kg. |
| Purity (Lead) |
Not more than 1 mg/kg. |